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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Compatibility testing - Antibody detection and identification - Blood components and storage - Blood group systems and antigens |
| Topic 2: Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology |
| Topic 3: Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Quality control and quality assurance |
| Topic 4: Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis |
| Topic 5: Hematology | 20-25% | - Red blood cell physiology and disorders - Routine and automated hematology procedures - White blood cell morphology and diseases - Hemostasis and coagulation testing |
| Topic 6: Immunology and Serology | 5-10% | - Immunologic testing methodologies - Immune system principles and components - Infectious disease serology - Autoimmune and immunodeficiency disorders |
| Topic 7: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. An international, nonprofit organization that establishes standards of best current practice for clinical laboratories is
A) Clinical Laboratory Standards Institute (CLSI)
B) Centers for Disease Control and Prevention (CDC)
C) the Joint Commission
D) Commission on Office Laboratory Accreditation (COLA)
2. Allergen-specific IgE, synthesized in response to allergens, becomes fixed to receptors on cellular membranes, especially those of basophils. If these receptor-bound IgE molecules are aggregated on re-exposure to specific allergen, both mast cells and basophils produce mediators that result in the allergic response. IgE-antigen interaction at the cell surface causes degranulation of cells and release of substances including: histamine, SRS-A, platelet activator, a kallikrein, and an eosinophil chemotactic factor. Basophils are the principal cells that bind IgE antibody while their number of receptor sites is proportional to serum IgE levels. Eosinophils are drawn to the site by the basophil chemotaxis mechanism, but are not the main cell which binds the IgE antibody.
Immunology
The mediator cells that bind MOST to IgE antibodies are:
A) Eosinophils
B) Polymorphonuclear neutrophils
C) Basophils
D) Macrophages
3. Following an accidental needlestick, the person should be tested for all of the following except:
A) hepatitis B virus (HBV)
B) human immunodeficiency virus (HIV)
C) hepatitis C virus (HCV)
D) hepatitis A virus (HAV)
4. The beta hemoglobin chain loci are found on which chromosome?
A) Chromosome 11
B) Chromosome 16
5. The activities conducted in a laboratory with a certificate of registration include:
A) physicians to perform microscopy only
B) urinalysis only
C) waived tests only
D) moderate- or high-complexity lab testing until the lab is determined by survey to be in compliance
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: C | Question # 3 Answer: D | Question # 4 Answer: A | Question # 5 Answer: D |






